肉桂醛对HL-60细胞的生长抑制及诱导凋亡作用研究

摘要

Objective： To determine the proliferation inhibitory and apoptosis-inducing effects of cinnamaldehyde on HL-60 cells.Methods： The proliferation inhibitory effect was evaluated using trypan-blue staining and MTT assay,and the apoptosis-inducing effect using flow cytometry,scanning electron microscope（SEM） and transmission electron microscope（TEM）.Results： Cinnamaldehyde had an oblivious inhibitory effect on the proliferation of HL-60 cells with dependence upon its concentration and treatment time and the LC50,median lethal concentration was 34.87 μg/mL.The rate of apoptosis in HL-60 cells was 32.6 % after being treated with cinnamaldehyde at the dose of 20 μg/mL.for 16 h.Typical apoptotic morphological changes in the cells was observed under fluorescence microscopy,SEM and TEM.Conclusion： cinnamaldehyde cna notably inhibit the proliferation and induce the apoptosis of HL-60 cells.%目的：探讨肉桂醛对HL-60细胞的增殖及凋亡的影响。方法：采用台盼蓝细胞计数和四甲基偶氮唑蓝（MTT）法研究肉桂醛对HL-60细胞增殖的抑制作用;采用流失细胞仪（FCM）、荧光显微镜（FM）、扫描电镜（SEM）以及透射电镜（TEM）探讨肉桂醛对HL-60细胞的诱导凋亡作用。结果：台盼蓝细胞计数和MTT法结果表明：肉桂醛对HL-60细胞的增殖有明显抑制作用,抑制作用与肉桂醛质量浓度及作用时间呈依赖关系,其半数致死质量浓度为34.87μg/mL;流式细胞仪检测结果表明：肉桂醛（终质量浓度20μg/mL）作用16h后,HL-60细胞早期凋亡比例为32.6%;该组细胞在荧光显微镜、扫描电镜以及透射电镜观察下均呈现早期凋亡特征。结论：肉桂醛能抑制HL-60细胞增殖并促进其凋亡。