沙棘叶水溶性多糖的抗突变作用

摘要

In order to explore the anti-mutagenic effect of water-soluble polysaccharides from Hippophae rhamnoides leaves（WPHL）,the inhibitory effect of WPHL on bone mouse marrow cell micronuclei,abnormal sperm and hepatic DNA damage induced by cyclophosphamide（CP） was studied.The results show that WPHL at the dose of 50 mg/kg·d significantly inhibited the frequency of micronucleus（P 0.05） and sperm abnormality（P 0.05）.In addition,WPHL could also significantly decrease the number and tail length of hepatic comet cells（P 0.05）.Therefore,WPHL has anti-mutagenic potential.%为了研究沙棘叶水溶性多糖（WPHL）的抗突变作用,本实验通过微核实验、精子畸变实验和彗星实验观察WPHL对环磷酰胺（CP）诱发的小鼠骨髓嗜多染红细胞微核、肝细胞DNA损伤和精子畸变的抑制作用。结果表明：WPHL在50mg/（kg.d）剂量时,对CP诱发的微核（P〈0.05）和精子畸变（P〈0.05）有明显的抑制作用,并可以显著减小肝脏彗星细胞的数量和缩短彗星细胞尾巴的长度（P〈0.05）。说明有显著的抗突变能力。