大孔吸附树脂吸附分离高活性玉米抗氧化肽

摘要

In this study,three types of macroporous resin were assessed for their adsorption and desorption capacity towards antioxidant peptides in the maize protein hydrolysate,prepared by alcalase hydrolysis,and XAD-7HP resin was found to be the most suitable resin among them.The static adsorption of XAD-7HP resin and the static desportion of antioxidant peptides with different desorption solvents were performed under different pH conditions.As a result,the optimal process parameters for the separation of antioxidant peptides were sample pH 7.0 and 70% aqueous ethanol as the desorption solvent.Antioxidant maize peptides were successfully separated by the dynamic adsorption of XAD-7HP resin,resulting in a 2-fold increase in DPPH free radical scavenging activity.%采用大孔吸附树脂对玉米抗氧化肽进行分离,通过单因素试验得出XAD-7HP树脂为最适树脂,在此基础上,研究不同pH值条件下的静态吸附能力和不同解吸剂的静态解吸能力等实验,确定玉米抗氧化肽吸附分离的基本参数为pH7.0、解吸剂体积分数70%。通过动态吸附分离实验得出,该树脂可以达到分离纯化玉米抗氧化肽的目的,而且分离后的玉米肽的抗氧化活性比原液提高了1倍。