中心组合设计-响应面分析法优化莽吉柿果皮中总氧杂蒽酮的超声提取工艺

摘要

A central composite design (CCD) was used to optimize the ultrasonic-assisted extraction of total xanthones from mangosteen pericarp. The effects of ethanol concentration, ultrasonication time and solvent-to-solid ratio as well as their interactions on the extraction rate of total xanthones were investigated. A predictive regression model was developed using the SAS software by response surface analysis and its reliability was validated. The optimal extraction conditions were 90.9%of ethanol concentration, 17.1:1 (mL/g) of solvent-to-solid ratio and 73.8 min of ultrasonication time as determined by ridge analysis, resulting in an average actual yield of total xanthones of 7.73%(n=3).%采用中心组合设计方法优化莽吉柿果皮中总氧杂蒽酮的提取工艺，研究乙醇体积分数、超声时间、液固比及其交互作用对总氧杂蒽酮提取率的影响。应用SAS软件和响应面分析相结合的方法，模拟得到回归方程的预测模型和可信度，并通过岭脊分析得到最佳的提取条件为乙醇体积分数90.9%、超声时间73.8min、液固比17.1：1(mL/g)。该条件下提取率为7.73%。