黄芩茎叶中总黄酮提取工艺优化

摘要

以黄芩茎叶为原料，采用醇提法提取黄芩茎叶总黄酮。在单因素试验的基础上，以提取温度、提取时间、乙醇体积分数和料液比为考察因素，采用L9(34)正交试验探讨醇提法提取黄芩茎叶总黄酮工艺。最佳工艺条件：以70%乙醇为溶剂、提取温度70℃、料液比1：18(g/mL)、提取3次、每次2.0h，此时黄芩茎叶提取物中总黄酮含量为21.02%，总黄酮得率1.39%。%The extraction of total flavonoids from stems and leaves of Scutellaria baicalensis Georgi by ethanol extraction was optimized using an L9(34) orthogonal array design. One-factor-at-a-time method was used to investigate the effects of temperature, extraction time, ethanol concentration and solid/solvent ratio on extraction efficiency. The optimum conditions for the extraction of total flavonoids were determined as three extraction cycles with 70% ethanol at a solid/solvent ratio of 1:18 (g/mL), 70 ℃ for 2.0 h each. Under these conditions, the yield and purity of crude total flavonoid extract were 21.02% and 1.39%, respectively.