荔枝皮原花青素低聚体的定性分析

摘要

Litchi （Litchi chinensis Sonn.） is a subtropical fruit of high commercial value; however, its pericarp results in a reduced market value. In the present study, oligomeric procyanidins from Litchi chinensis pericarp （LPOPC） were prepared and qualitatively analyzed. LPOPC were extracted with ethanol aqueous solution, purified by AB-8 macroporous resin chromatography, and fractionated by Toyopearl HW-40S resin chromatography to obtain LPOPC. LPOPC was found to comprise three fractions with different degrees of polymerization （DP）. The results of FT-IR, HPLC and LC-MS analyses revealed that the major components of LPOPC were （-）-epicatechin, procyanidin A2, procyanidin A3. Highly pure monomer, dimmers and trimers were obtained through selective collection of fractions.%制备并定性分析荔枝皮原花青素低聚体（LPOPC）。荔枝皮原花青素（LPPC）通过乙醇浸提，AB-8大孔树脂纯化，ToyopearlHW-40S树脂层析分级处理得到LPOPC，并按聚合度分成了3个组分。通过红外光谱、高效液相色谱（HPLC）和液相色谱．质谱联用（LC—MS）等分析表明，LPOPC中主要成分为（一）-表儿茶素，并且含有A型原花青素二聚体和A型原花青素三聚体。通过选择性的级分收集，可以得到纯度较高的原花青素单体、二聚体和三聚体。