混菌固态发酵生产菜籽肽工艺条件优化

摘要

以肽得率、氮溶解指数和硫苷降解率为指标,通过单因素试验初步得到枯草芽孢杆菌与雅致放射毛霉混菌固态发酵生产菜籽肽的发酵条件,再根据Box-Behnken中心组合试验设计,在单因素试验的基础上,采用响应面分析法进一步优化混菌发酵条件,确定最佳工艺条件为：枯草芽孢杆菌与雅致放射毛霉同时接种,两菌接种体积比4：1、发酵温度32.1℃、发酵时间4.64d,此条件下菜籽肽得率为9.85%,硫苷降解率为67.83%。%Based on peptide yield,nitrogen solubility index（NSI） and glucosinolate degradation rate,the production conditions for rapeseed peptide by mixed solid-state fermentation using Bacillus subtilis and Actinomucor elegans preliminarily determined by one-factor-at-a-a-time method and further optimized by response surface analysis coupled with Box-Behnken design.The optimal fermentation conditons were 4：1 of Bacillus subtilis-to-Actinomucor elegans ratio,32.1 ℃ of fermentation temperature,4.64 d of fermentation time,resulting in a peptide yield of 9.85% and a glucosinolate degradation rate of 67.83%.