单增李斯特氏菌MALDI-TOF-MS鉴定与分型研究

摘要

In order to establish a matrix-assisted laser desorption ionisation time-of-flight mass spectrometry（MALDI-TOF-MS） method for the rapid identification and subtyping of Listeria monocytogenes,37 Listeria monocytogenes isolates were tested using MALDI-TOF-MS and characteristic protein fingerprints from their spectra were acquired and summarized into standard spectra to construct a database for the identification of Listeria monocytogenes.The method was highly reliable as demonstrated by the results obtained by the method for standard Listeria monocytogenes strains.Based on the identification database constructed,the Listeria monocytogenes isolates were classed into 9 subtypes at the protein level.%为建立单增李斯特氏菌的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）快速鉴定与分型方法,实验收集37株单增李斯特氏菌分离株,应用MALDI-TOF-MS采集图谱,获取独特的蛋白质指纹图谱,汇总成标准图谱,建立单增李斯特氏菌鉴定数据库。采用单增李斯特氏菌标准菌株进行验证,表明鉴定结果的可信度很高。在数据库信息的基础上,对37株单增李斯特氏菌分离株进行聚类分型。分型结果表明,在蛋白质水平上,MALDI-TOF-MS可把37株单增李斯特氏菌分成9个型别。