采用水蒸气蒸馏法提取采自西藏地区藏药大籽蒿的精油，通过气相色谱-质谱联用技术分析鉴定其化学成分，采用峰面积归一化法确定各成分的相对含量；并对大籽蒿精油体外抑菌能力和抗氧化能力进行测定。大籽蒿精油中共鉴定48种化合物，占精油总量的95.36%，主要成分为红没药醇、兰香油奥、α-水芹烯、棕榈酸和薰衣草醇等；体外抑菌结果表明，大籽蒿精油对枯草芽孢杆菌、粪肠球菌、金黄色葡萄球菌和新型隐球菌具有较强的抑菌效果，而对大肠杆菌具有较弱抑菌效果；体外抗氧化活性结果表明大籽蒿精油对1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基和羟自由基具有一定的的清除能力，且清除效果强于VC。%The chemical composition, antimicrobial and antioxidant activities of the essential oil of Artemisia sieversiana, a traditional medicinal herb in Tibet, China, were investigated. A total of 48 components, representing 95.36% of the total oil, were identified by gas chromatography-mass spectrometry (GC-MS), with the main constituents being α-bisabolol, chamazulene, α-phellandrene, palmitic acid and lavandulol. The antimicrobial activities of the oil were evaluated against five microorganisms, Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and Crytococcus neofonman, respectively. The results showed that the oil possessed inhibitory activity against B. subtilis, E. faccalis, S. aureusand C. neoformans, amon+g which the best inhibition was observed against C. neoforman with a minimum inhibitory concentration (MIC) of 5.5 μg/mL. The oil revealed a relatively stronger scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+·) and hydroxyl free radicals in a concentration-dependent way.
展开▼
