菠萝蜜多糖对脾淋巴细胞抗氧化作用及免疫功能的影响

摘要

本研究旨在探明菠萝蜜多糖(polysaccharide from jackfruit pulp,JFP-Ps)对正常小鼠脾淋巴细胞增殖和免疫调节的影响.通过体外小鼠脾淋巴细胞的培养实验,测定不同质量浓度JFP-Ps对正常小鼠脾淋巴细胞增殖、抗氧化能力(总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力和丙二醛(malonic dialdehyde,MDA)含量)及细胞因子(肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素-γ (interferon-γ,IFN-γ)和白细胞介素-1β(interleukin-1β,IL-1β))分泌水平的影响.结果显示,JFP-Ps可促进小鼠脾淋巴细胞增殖,质量浓度为80 μg/mL和160 μg/mL时效果显著(P<0.05);当JFP-Ps质量浓度为40 μg/mL甚至更高时,T-AOC及GSH-Px、SOD和CAT活力显著升高(P<0.05),但MDA含量显著降低(P<0.05);JFP-Ps在质量浓度40~160 μg/mL范围内可显著促进脾淋巴细胞TNF-α、IFN-γ和IL-1β的分泌(P<0.05).结果表明JFP-Ps的免疫增强活性可能与其促进小鼠脾淋巴细胞增殖、提高抗氧化活性及诱导细胞因子TNF-α、IFN-γ和IL-1β分泌水平有关.%The antioxidant and immunomodulatory effects of water-soluble polysaccharide from jackfruit (Artocarpus heterophyllus Lam.) pulp (JFP-Ps) were investigated.The effect of JFP-Ps on the proliferation of normal mouse spleen lymphocytes, antioxidant capacity (total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activity) and malonic dialdehyde (MDA) contents, as well as the secretion of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) were detected by culturing mouse spleen lymphocytes in vitro in the presence of different concentrations of JFP-Ps. The results showed that JFP-Ps at 80 and 160 μg/mL significantly (P < 0.05) increased lymphocyte proliferation, and it at 40 μg/mL and above enhanced T-AOC, GSH-Px, SOD and CAT activities and reduced MDA content. Moreover, JFP-Ps at 40, 80 and 160 μg/mL significantly increased the secretion of TNF-α, IFN-γ and IL-1β in spleen lymphocytes (P < 0.05). These results indicate that JFP-Ps exerts immunomodulatory effect likely by inducing lymphocyte proliferation, enhancing antioxidant activity and increasing the secretion of TNF-α, IFN-γ and IL-1β.