巴莲莲子生物碱减弱盐酸/乙醇诱导的小鼠胃黏膜损伤研究

摘要

本研究的目的是探讨巴莲莲子生物碱对盐酸/乙醇诱导的胃黏膜损伤的作用并对其机制进行研究.小鼠被巴莲莲子生物碱(alkaloids from Ba lotus seed,ABLS)灌胃处理后用盐酸/乙醇诱导胃黏膜损伤,小鼠的血清细胞因子及其他指标采用试剂盒进行测定,同时用逆转录聚合酶链式反应和western blot对胃组织进行检测.100 mg/(kg·d)的ABLS处理小鼠的胃黏膜损伤面积、胃损伤抑制率、胃液分泌量和pH值分别为1.13 mm2、84.5％、0.52 mL和3.3.ABLS作用的小鼠血清白细胞介素(interleukin,IL)-6、IL-12、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、γ-干扰素(interferon-γ,IFN-γ)、胃动素(motilin,MOT)、P物质(substance P,SP)、内皮素-1(endothelin-1,ET-1)水平低于对照组小鼠,而生长抑素(somatostatin,SS)、血管活性肠肽(vasoactive intestinal peptide,VIP)水平高于对照组.ABLS作用小鼠胃组织比对照组小鼠具有更高的超氧化物歧化酶(superoxide dismutase,SOD)活性和更低的MDA含量.相比对照组ABLS处理还可以上调胃组织中核因子κB抑制蛋白α(inhibitor kappaB-α,IKB-α)、表皮生长因子(epidermal growth factor,EGF)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、神经元型一氧化氮合酶(euronal nitric oxide synthase,nNOS)、内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)、Mn-SOD、Gu/Zn-SOD、过氧化氢酶(catalase,CAT)的mRNA和蛋白表达,同时下调核因子κB (nuclear factor kappaB,NF-κB)、诱导型一氧化氮合成酶(inducible nitric oxide synthase,iNOS)表达.这项研究表明ABLS处理可减轻胃黏膜损伤,并且这种作用是来自ABLS的抗氧化能力.%The aim of this study was to investigate the effect of alkaloids from Ba lotus seeds (Nelumbo nucifera Gaertn) on HC1/ethanol-induced gastric mucosal injury and to elucidate the underlying mechanism.Mice were intragastrically administered with alkaloids from Ba lotus seed (ABLS) and gastric mucosal injury was induced in mice by HC1/ethanol.Serum cytokine and other index levels were examined by using commercial kits,and the gastric tissue was determined by reverse transcription polymerase chain reaction (RT-PCR) and western blot assay.The gastric mucosal injury area,percentage inhibition,gastric secretion volume and pH of gastric juice in the 100 mg/(kg·d) ABLS treated-mice were 1.13 mm2,84.5％,0.52 mL and 3.3,respectively.Additionally,serum levels of interleukin (IL)-6,IL-12,tumor necrosis factor-α (TNF-α),interferon-γ (IFN-γ),motilin (MOT),substance P (SP) and endothelin-1 (ET-1) in these mice were lower than those in the control mice,while the levels of somatostatin (SS) and vasoactive intestinal peptide (VIP) were higher than those in the control mice.The treated mice had higher superoxide dismutase (SOD) activity,and lower MDA level in gastric tissues than the control ones.ABLS also could increase the mRNA and protein expression levels of inhibitor kappaB-α (Iκ B-α),epidermal growth factor (EGF),epidermal growth factor receptor (EGFR),neuronal nitric oxide synthase (nNOS),endothelial NOS (eNOS),Mn-SOD,Gu/Zn-SOD and catalase (CAT),and decrease nuclear factor kappaB (NF-κB) and inducible NOS (iNOS) expression in gastric tissues compared to those in the control group.This study has demonstrated that ABLS treatment attenuated gastric mucosal injury due to their antioxidant capacity.