黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响

摘要

目的:研究水溶性黑灵芝多糖(a water-soluble polysaccharides from Ganoderma atrum,PSG)-1体内抗炎活性及对甘露糖受体(mannose receptor,MR)表达的影响.方法:腹腔注射脂多糖(lipopolysaccharide,LPS)建立小鼠体内炎症模型,随机分为5组:正常对照组、LPS组、PSG-1(高、中、低剂量,分别为100、50、25 mg/ (kg·d))+LPS组.流式细胞仪检测巨噬细胞中MR表达、吞噬功能及活性氧(reactiveoxygen species,ROS)生成,用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)分析血清中肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-1β和IL-6的含量.结果:与正常对照组相比,LPS组腹腔巨噬细胞表面MR表达量下降,与LPS组相比,PSG-1+LPS组腹腔巨噬细胞表面MR表达量极显著增加(P＜0.01);与正常对照组相比,LPS组腹腔巨噬细胞吞噬能力和ROS生成增加,与LPS组相比,PSG-1+LPS组腹腔巨噬细胞吞噬功能和ROS生成显著降低(P＜0.05,P＜0.0l);与正常对照组相比,小鼠经LPS处理后,血清中TNF-α、IL-1β和IL-6的分泌水平极显著升高(P＜0.01);与LPS组相比,小鼠灌胃PSG-1后,PSG-1(高剂量)+LPS组中TNF-α的含量显著降低(P＜0.05),IL-1β和IL-6的分泌水平无显著差异.结论:PSG-1具有抗炎作用,可部分抑制LPS诱导的体内炎症,其机理与PSG-1促进小鼠腹腔巨噬细胞表面MR的表达、抑制巨噬细胞向M1型极化有关.%Objective:To explore the in vivo anti-inflammatory activity of a water-soluble polysaccharide purified from Ganoderma atrum,named PSG-1,and its effect on mannose receptor (MR) expression.Methods:In the present study,lipopolysaccharide (LPS) via intraperitoneal injection was applied to establish a mouse model of inflammation.Mice were randomly divided into five groups:control group,LPS group,high-dose PSG-1 (100 mg/(kg·d)) + LPS group,mediumdose PSG-1 (50 mg/(kg·d)) + LPS group and low-dose PSG-1 (25 mg/(kg·d)) + LPS group.MR expression,phagocytosis and the level of reactive oxygen species (ROS) in maerophages were analyzed by flow cytometry.The levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum were determined by enzyme-linked immunosorbent assay (ELISA).Results:Compared with the control group,MR expression on the macrophage surface was decreased in the LPS group.In contrast,MR expression on the macrophage surface was significantly increased in the PSG-1 + LPS group in comparison with the LPS group (P ＜ 0.01).Macrophage phagocytosis and the level of ROS in the LPS group were higher than in the control group,which were significantly decreased after administration of PSG-1 (P＜0.05,P＜0.01).After mice were administrated with LPS,the levels of TNF-α,IL-1β and IL-6 were significantly increased in serum in comparison with the control group (P ＜ 0.01).Compared with the LPS group,the expression level of TNF-α was significantly decreased after administration of high-dose PSG-1 (P ＜ 0.05),but the expression levels of IL-1β and IL-6 did not significantly change.Conclusion:PSG-1 had an anti-inflammatory effect,being able to partly suppress inflammation induced by LPS in vivo,by promoting MR expression on the peritoneal macrophage surface in mice and suppressing macrophage polarization to M1 phenotype.