Lotus seedpod oligomeric procyanidin (LSOPC)-loaded nanoliposome was prepared by reverse-phase evaporation method in combination with ultrasonic treatment. The preparation process was optimized and the stability and antioxidant activity of the nanoliposome were determined in this research. The results indicated that the optimal parameters of the preparation process were as follows:ratio of phosphatidylcholine and cholesterol, 3:1; LSOPC concentration, 0.33 mg/mL;and Tween 80 concentration, 16.7 mg/mL. The average particle size of the nanoliposome prepared under these conditions was (35.57 ± 0.08) nm, and the polydispersity index was 0.153 ± 0.01. The highest encapsulation efficiency of (71.97 ± 0.42)% was achieved at an LSOPC concentration of 1%. The spherical vesicle structure was observed by transmission electron microscopy, and the nanoliposome was found to be stable at low temperature. Low concentrations of glucose and sucrose had little impact on the size of LSOPC nanoliposome. Fe3+, Cu2+, Zn2+, and Pb2+affected its size. Compared with the nanoliposome, the 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt scavenging activity and ferric reducing power of LSOPC solution dropped significantly after being stored at 4 ℃ for a week. LSOPC nanoliposome had higher total antioxidant capacity, as measured by oxygen radical absorbance capacity (ORAC), than LSOPC solution.%采用逆向蒸发法和超声处理相结合成功地制备莲房原花青素低聚体(lotus seedpod oligomeric procyanidins,LSOPC)纳米脂质体,优化制备工艺并考察其稳定性和抗氧化活性.结果表明,LSOPC纳米脂质体的最佳制备工艺参数为大豆卵磷脂-胆固醇质量比3:1、LSOPC添加量0.33?mg/mL、吐温80添加量16.7?mg/mL.在此条件下,LSOPC纳米脂质体的平均粒径为(35.57±0.08)nm,多分散指数为0.153±0.01.当LSOPC载量为1%时,脂质体为最高包埋率(71.97±0.42)%.LSOPC纳米脂质体在透射电子显微镜观察下为球状的囊泡结构,在低温时比较稳定.低浓度的葡萄糖、蔗糖及防腐剂对LSOPC纳米脂质体的粒径无显著性影响,而金属离子Fe3+、Cu2+、Zn2+、Pb2+显著影响其粒径.在4?℃贮藏7?d后,与脂质体相比,LSOPC溶液清除2,2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基和铁还原能力显著下降.总抗氧化能力测定结果说明,LSOPC纳米脂质体的总抗氧化能力优于LSOPC溶液.
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