南瓜、山药、葛根和桑叶提取物复方对四氧嘧啶模型小鼠的降血糖作用

摘要

Objective:The objective of this research was to study the hypoglycemic effect of the combined extract of pumpkin,yam,kudzuvine root and mulberry leaves on alloxan-induced diabetic mice.Methods:A diabetic mouse model was induced by alloxan.Another 10 mice served as a normal group.The model diabetic mice were randomly divided into model group,positive group (acarbose,200 mg/kg mb) and low and high dose treatment groups (12 and 36 g dry raw material/kg mb).After intragastric administration for 4 weeks,we studied the effect of the combined extract on the body weight,food and water intake,fasting blood glucose level,glycogen,serum insulin,glycosylated serum protein (GSP),glycosylated hemoglobin (GHb) and antioxidant status of diabetic mice.Results:The major bioactive components of the combined extract were pumpkin polysaccharide,yam polysaccharide,and puerarin at levels of (0.93 ± 0.04),(1.01 ± 0.06),and (0.28 ± 0.01) g/100 g dry raw material,respectively.Compared with the model group,the body weight and glycogen content of mice in the intervention group were significantly improved,while the food and water intake,fasting blood glucose,GSP and GHb were decreased significantly.The insulin sensitivity was also improved.The hypoglycemic effect of the high dose group was better than that of the low dose group.Meanwhile,compared with the model group,the total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity in the liver of mice administered with the combined extract were increased,while the content of malondialdehyde (MDA) was decreased.Thus,it was suggested that oxidative stress in diabetic mice was improved by administration of the combined extract.Conclusion:The combined extract of pumpkin,yam,kudzuvine root and mulberry leaves can effectively reduce the blood glucose level of alloxan-induced diabetic mice and improve oxidative stress.%目的:研究南瓜、山药、葛根和桑叶提取物复方对四氧嘧啶糖尿病模型小鼠的降血糖作用.方法:以四氧嘧啶诱导建立糖尿病小鼠模型,实验保留10只小鼠作为正常对照组,并将建模成功的小鼠随机分为模型组、阳性组(阿卡波糖,200 mg/kg mb)、植物提取物复方低、高剂量组(12、36 g/kg mb,以原料干质量计).连续灌胃给药4周,研究植物提取物复方对糖尿病小鼠体质量及饮食饮水量、空腹血糖浓度、肝糖原含量、糖化血清蛋白(glucosylated serum protein,GSP)浓度、糖化血红蛋白(glycosylated hemoglobin,GHb)相对含量和抗氧化指标的影响.结果:植物提取物复方中的主要有效成分为南瓜多糖、山药多糖和葛根素,含量分别为(0.93±0.04)、(1.01±0.06)、(0.28±0.01) g/1009(以原料干质量计).干预4周后,与模型组相比,植物提取物复方组小鼠体质量、肝糖原含量显著增加,饮食饮水量、空腹血糖浓度、GSP浓度和GHb相对含量明显降低,胰岛素敏感性增强,且高剂量效果优于低剂量.植物提取物复方组较模型组总抗氧化能力和超氧化物歧化酶活力明显升高,丙二醛含量显著降低,表现出改善小鼠氧化应激的作用.结论:南瓜、山药、葛根和桑叶提取物复方能够显著降低四氧嘧啶性糖尿病模型小鼠血糖浓度,有效改善氧化应激状态.