气相色谱/质谱法测定蔬菜中44种农药残留

摘要

A rapid,simple and sensitive method was developed for the simultaneous determining of pesticide residues in vegetables.The target pesticides in vegetables were purified by PSA powder and graphon dispersive sorbent.The drugs were analysed by GPC-GC/MS,which removed most of macromolecule such as pigment and fat.For the 44 pesticide residues in level of 0.008 mg/kg-0.064 mg/kg,the average recoveries (n=5)were in range of 65%-120%and the RSD was less than 15%.The limits of detection were in range of 0.000 5 mg/kg-0.2 mg/kg.The method in this study could be used for detecting the pesticide residues in vegetables.%建立了蔬菜中多种农药残留同时检测的分析方法。样品中残留农药采用乙腈提取，使用PSA粉和石墨炭黑吸附剂净化，再经在线凝胶渗透色谱-气相色谱/质谱（GPC-GC/MS）分析，进一步除去样液中色素和脂肪等大分子干扰物质。加标水平为0.008 mg/kg~0.064 mg/kg时，回收率为65%~120%，相对标准偏差小于15%；44种农药的检出限为0.0005 mg/kg~0.02 mg/kg。实验证明，该方法操作简单快速、准确度高，能够满足蔬菜中多种农药残留限量标准的检测要求。