香椿组织培养技术研究

摘要

以野生香椿种子为外植体进行培养，将香椿种子用75%酒精溶液消毒20 s，再用0.1%升汞溶液消毒5 min，无菌水冲洗4次～6次，接种到培养基上。在接种8 d～10 d后外植体分化出带有一片小叶的新生枝。切取子叶和下胚轴作为外植体接种B5+蔗糖30 g/L的培养基中，10 d后形成增殖苗。通过组织培养技术，缩短香椿的生长周期，再将香椿的嫩芽做如下处理浸泡（料液比1∶4，0.05%碳酸钠）、清洗、浸烫（95℃水浴2 min，500 mg/kg的葡萄糖酸锌溶液）、沥干冷却、切碎、拌料、加入添加剂、装罐、杀菌（85℃，30 min）、冷却、贮藏。%This topic is based on the seeds of wild toon were cultured as explants, the toon seeds with 75%alcohol solution to disinfect 20 s, and then 0.1%mercuric chloride solution In disinfection 5min, the sterile water 4 to 6 times, inoculated into the medium. Explants differentiation after inoculated 8 d-10 d, with a lobular new branches. Cut to take cotyledon and hypocotyl as explants inoculated B5+sucrose 30 g/L medium, after 10 days form the proliferation of seedlings. By tissue culture techniques to shorten the growth cycle of the toon , and then toon buds do the following processing immersion (1∶4,0.05%sodium carbonate)-Cleaning-scalding liquid ratio (95℃water bath for 2 min, 500 mg/kgglucose solution of zinc)-drain the cooling-chopped-spices-mix in additives-canning-sterilization (85 ° C, 30 min)-cooling-storage.