伽师瓜超氧化物歧化酶cDNA的克隆与分析

摘要

以“卡拉克塞”伽师瓜为试验材料，采用甜瓜基因组辅助设计超氧化物歧化酶（Superoxide Dismutase，SOD）基因扩增引物。以伽师瓜cDNA为模板克隆超氧化物歧化酶基因CDS区序列，获得三条超氧化物歧化酶基因序列，分别命名为JSGSOD1（Genebank登录号KT160023）、JSGSOD2（Genebank登录号KT160024）和JSGSOD3（Genebank登录号KT160025），序列长度分别为987 bp、804 bp和459 bp。伽师瓜SOD与甜瓜SOD具有高度的同源性。JSGSOD1含CuSOD结构域，JSGSOD2含FeSOD家族结构域，而JSGSOD3含Cu/ZnSOD家族结构域。并利用相关软件对其理化性质分析和亚细胞器定位进行了预测分析。%Melon genome was used to design superoxide dismutase(SOD) gene amplification primers. Three su-peroxide dismutase gene sequences obtained from“kalakesai”Jiashi melon cDNA were named as JSGSOD1 (Genebank accession number:KT160023),JSGSOD2(Genebank accession number:KT160024) and JSGSOD3 (Genebank accession number:KT160025), sequence lengths were 987 bp,804 bp and 459 bp,respectively. Jiashi melon SOD and melon SOD had a high degree of homology. JSGSOD1 contained CuSOD family domain, JSGSOD2 contained FeSOD family domain, JSGSOD3 contained Cu/ZnSOD family domain. The paper also an-alyzed its physical and chemical properties and subcellular localization analysis with relevant software.