鲢鱼鱼皮中酸溶性胶原蛋白提取工艺研究

摘要

With silver carp fish skin as raw material,the optimal extraction parameters of acid-soluble collagens were explored by single factor test and orthogonal test. The denaturation temperatures of acid-soluble collagens were analyzed by AR-500 dynamic rheometer. The results showed that the optimal parameters for the extraction of acid-soluble collagens from silver carp skin were with acetate as the extraction agent,the concentration of acetic acid 0.3 mol/L,material/liquid ratio of 1∶60 (g/mL) for 96 h. The starting denaturation temperature of acid-soluble collagens from silver carp skin was 32.31℃,and the peak temperature was 34.82℃.%以鲢鱼鱼皮为原料，通过单因素试验和正交试验对鲢鱼鱼皮中酸溶性胶原蛋白的提取工艺参数进行分析，并用AR-500动态流变仪对鲢鱼鱼皮中酸溶性胶原蛋白的热变性温度进行分析。结果表明：鲢鱼鱼皮原料中酸溶性胶原蛋白提取的最佳工艺条件为：以乙酸作为提取剂，乙酸浓度0.3 mol/L，料液比1∶60（g/mL），提取时间96 h；此胶原蛋白的热变性起始温度为32.31℃，峰值温度为34.82℃。