INCORPORATION OF ADENINE-4, 6-C114 INTO ACID-SOLUBLE NUCLEOTIDES IN THE C57 MOUSE

摘要

Adenine-4, 6-C14 has been administered to male C57 mice, and the distri¬bution of the radioactivity has been studied at short time intervals after admin¬istration, particularly in the cold TCA-soluble fraction. It has been shown that adenine is rapidly incorporated into the nucleotides of tissue, including 5'-adenylic acid, adenosine diphosphate, adenosine triphosphate, the corre¬sponding guanylic acid derivatives, dephosphopyridine nucleotide, inosinic acid, and possibly TPN. The adenine is rapidly equilibrated between AMP, ADP, and ATP, and is less rapidly incorporated into DPN. The specific activity of the guanine nucleotides is always considerably less than that of the adenine nucleotides. Evidence is presented that the TGA-soluble fraction serves as a precursor for RNA and DNA.