一测多评法测定保健食品中6种大豆异黄酮类成分

摘要

A quantitative analysis method of multi-components with single marker (QAMS) was established and validated to simultaneously determine six Soybean isoflavones (ISO)in health foods , which can solve the dilemma of natural chemical reference substances are expensive and unavailable. The samples were ultrasonic-assisted extracted with methanol, the column was Waters XbridgeC18(150 mm×4.6 mm,3.5μm),using 2%acetic acid and acetonitrile as the mobile phase with gradient elution, 260 nm and with DAD detection was used. The component to be measured were confirmed by DAD spectrum, reference chromatogram and the relative re-tention time of genistin. The relative correction factors (RCF) of the other five soybean isoflavones were deter-mined within the linear ranges, the RCF had a good reproducibility in various instruments, chromatographic columns(RSD=0.4%-3.5%). According to their RCF, six ISO in health food could be quantified.The results of QAMS method were validated by comparing with that of external standard method by quantified three different sources of health food , and the relative average deviation of the two method were 0.3%-5.8%. The method can be used for the quantitative analysis and quality evalution for ISOs and its related health food.%建立一测多评方法实现只用一个对照品同时测定保健食品中6种大豆异黄酮类成分的含量，以解决含大豆异黄酮类成分保健食品对照品昂贵不易获得的难题。样品经甲醇超声波辅助提取后，采用Waters XbridgeC18（150 mm×4.6 mm，3.5μm）色谱柱，以2%乙酸和乙腈为流动相进行梯度洗脱，260 nm并辅以DAD紫外检测器检测。通过5种待测成分与染料木苷的相对保留时间、DAD光谱及参照图谱完成待测成分确认；通过研究确立其他5种待测成分与染料木苷的校正系数进行含量测定，一定线性范围内得到不同浓度对照品在不同仪器、人员及色谱柱上的校正系数RSD为0.4%~3.5%；3批不同来源保健食品中6种大豆异黄酮类成分测定结果与常规外标法所得结果结果偏差为0.3%~5.8%；所建立的一测多评方法可用以大豆异黄酮及其相关保健食品的定量分析及质量评价方法。