食品中克罗诺杆菌的分离和鉴定

摘要

利用国标方法对食品（蔬菜，熟食和豆制品）进行了克罗诺杆菌的污染情况调查及其分离菌株的鉴定；利用fusA基因对分离的克罗诺杆菌进行了遗传多样性分析，将克罗诺杆菌属的分离株鉴定到种的水平。主要取得了以下结论和认识：①39份食品（蔬菜23份，熟食14份和豆制品2份）中有14份样品检出了克罗诺杆菌，检出率为35.9%。蔬菜类检出8份（34.8%），熟食制品检出4份（28.6%），2份豆制品都检出（100.0%）；②对14株克罗诺杆菌分离株进行基于fusA基因的系统进行分析，结果显示这些分离株包括C. Sakazakii和C. malonaticus 2个种，其中C. sakazakii有9株， C. malonaticus有5株。%The method of national standard was used to detect the occurrence of Cronobacter spp. From food (milk, vegetables, cooked and soy) and further identified the isolation strain. FusA gene was used to analyze the genetic diversity of separated Cronobacter spp., which can identify species. This paper mainly has made the following conclusions and cognition: ①There are 39 other foods (23 vegetables, 14 cooked food and 2 of soy product) test too, 14 of which detected Cronobacter spp., in a rate of 35.9%. Vegetables were detected in 8 (34.8%), food products were detected in 4 (28.6%), 2 copies of soy products are detected (100.0%).②Based on fusA gene, the genetic diversity was analyzed for 14 Cronobacter spp., the result indicated that there are 9 C. sakazakii, and 5 C. malonaticus among these isolates.