茯苓皮三萜抑制酪氨酸酶机理研究

摘要

In order to investigate the healthgiving benefit of triterpenoids in Poria cocos peel on the inhibition a-gainst tyrosinase and enhance both the applications of Poria peel in production and processing and its added val-ue, the inhibition of triterpenoids from Poria cocos peel against tyrosinase was tested using in vitro biochemical enzymatic method and its mechanism was explored initially. The results showed that the inhibitory effect of chlo-roform extract on tyrosinase was the best and chloroform was a good extraction agent;meanwhile the inhibition was irreversible.%为明确茯苓皮中天然的三萜化合物抑制酪氨酸酶的保健功效，增强茯苓皮在生产加工中的应用，提高其附加价值，采用体外生化酶学法研究茯苓皮三萜化合物对酪氨酸酶活性的抑制作用，初步探究茯苓皮三萜对酪氨酸酶的抑制的机理。结果表明氯仿萃取物对酪氨酸酶的抑制效果最好，氯仿是良好的萃取剂；茯苓皮三萜对酪氨酸酶的抑制属于不可逆抑制。