黑果腺肋花楸黄酮提取工艺优化及外抗氧化研究

摘要

Black fruit A ronia as raw material,single factor and response surface analysis black fruit A ronia the extraction process,and Vc as control its antioxidant capacity and 1,1-diphenyl-2 trinitrobenzene hydrazine radical (DPPH·),superoxide anion radical (O2-·),hydroxyl radical (·OH) scavenging were studied.The results showed that the optimum parameters for the ethanol concentration 77.67 ％,extraction time 81 min,1 ∶ 20 (g/mL),in this condition,black fruit Aronia flavonoids yield an average of 5.14 ％.Effect of black fruit Aronia yield of flavonoids descending factor ethanol concentration＞ extraction time＞ solid-liquid ratio.The optimal parameters extracted black fruit A ronia flavonoids on · OH,O2-·,DPPH radical IC50 were 3.27,3.96,3.79 mg/mL,the maximum clearance rate of 29.58 ％,70.23 ％,61.41％ and showed a strong reducing power,has strong antioxidant activity in vitro.%应用黑果腺肋花楸作为原料,采用响应面法优化黑果腺肋花楸黄酮提取工艺,并利用VC做对照试验,对其还原力及以及·OH、02-·、DPPH自由基清除力进行研究.结果表明,最佳工艺参数为乙醇浓度77.67％、提取时间81 min、料液比1∶20(g/mL),在此条件下,黑果腺肋花楸黄酮得率5.14％.影响黑果腺肋花楸黄酮得率因素由大到小依次为乙醇浓度＞提取时间＞料液比.最佳工艺参数提取的黑果腺肋花楸黄酮对·OH、O2-·、DPPH自由基的IC.分别为3.27、3.96、3.79 mg/mL,最大清除率达29.58％、70.23％、61.41％,并且其还原能力强,能够说明其体外抗氧化活性较强.