紫外线等离子体复合诱变解淀粉芽孢杆菌提高NAG产量

摘要

To improve the N-acetyl-D-glucosamine (NAG) production of Bacillus amyloliquefaciens BI2 preserved by our laboratory, mutant strains had been screened with Ultraviolet-plasma composite mutagenesis through the multifunction plasma mutagenesis system(MPMS). Under the condition of plasma treatment at 15 s and ultraviolet treatment at 15 s, a genetically stable mutant BH-3-2 was screened out by glucose screening plate and flask fermentation culture. The NAG yield of BH-3-2 was 1.23 g/L, 4.1 times higher than that of BI2. Comparing the effect of plasma, ultraviolet and ultraviolet-plasma treatments on lethality rate and positive mutagenesis rate, the positive mutagenesis rate of composite mutagenesis was the highest 28％, which was 1.86 times that of plasma mutagenesis and 2.8 times that of Ultraviolet mutagenesis. As a result, Ultraviolet-plasma composite mutagenesis can be used as an efficient mutagenesis method.%为提高解淀粉芽孢杆菌产N-乙酰-D-氨基葡萄糖(N-acetyl-D-glucosamine,NAG)的能力,以实验室保藏的解淀粉芽孢杆菌BI2(B.amyloliquefaciens BI2)为出发菌株,选用等离子体和紫外双重复合诱变,在等离子体处理15 s和紫外处理15 s的复合诱变条件下,经葡萄糖筛选平板初筛以及摇瓶发酵复筛,筛选出一株遗传稳定的突变株BH-3-2,NAG产量可达1.23 g/L,较出发菌株提高4.1倍.比较等离子体、紫外单独诱变以及复合诱变效果,结果显示复合诱变正突变率最高,达到28％,是等离子体单独诱变的1.86倍,是紫外单独诱变的2.8倍,较单因素诱变正突变率提高明显,可以作为一种高效的诱变手段.