对提取分离的黑木耳多糖进行羧甲基化,并对人体肝癌HepG2细胞株进行干扰研究,探讨其增殖抑制作用.将HepG2细胞进行贴壁培养,对处在对数生长期的HepG2细胞进行对照试验.结果表明,黑木耳中性多糖组、黑木耳酸性多糖组对HepG2细胞毒试验得到细胞存活率50%时的药物浓度范围在200～600 μg/mL.两种未经羧甲基化改性的黑木耳多糖在体外对人HepG2细胞具有抑制作用,而经羧甲基化改性的黑木耳多糖则没有.%Cancer has been a challenge for humanity.Many scientific researchers have been exploring antitumor activities of some natural products.It was reported that polysaccharide had good antitumor activities.This paper reports on a water-soluble polysaccharide (AAP) and in how it was ultrasound-assisted and extracted from Auricularia auricular and fractioned by Cetyl Trimethyl Ammonium Bromide (CTAB).Then these two carboxymethylated polysaccharides (acidic polysaccharide,AAAP and neutral polysaccharide,NAAP) were prepared.Additional further explanation is given as to their structural characteristics by Fourier transform infrared spectrometer(FT-IR).And then inhibition of on the human HepG2 cell proliferation was studied.The results showed toxicity experiments 50％ viability of concentrations between the neutral polysaccharide from Auricularia and acidic polysaccharide from Auricularia on HepG2 cell was range 200 to 600 μg/mL.These two polysaccharides from Auricularia which have the potential medicinal value in inhibiting on human HepG2 cells in vitro, are well worth further studying.
展开▼
