苦瓜渣蛋白提取工艺优化及其体外抑制K562细胞增殖作用研究

摘要

采用单因素结合均匀设计优化了苦瓜渣蛋白盐溶法提取工艺,并对蛋白粗提物进行了体外抑制K562细胞增殖能力测定.结果表明,最优工艺条件为:盐浓度0.25 mol/L、时间3.59 h、pH为9、液料比10.36∶1(mL/g)、温度25℃、提取2次.该条件下实际提取率为9.28％.在试验浓度范围内,苦瓜渣粗蛋白对K562细胞生长具有明显的抑制作用,并呈量效关系.%Single factor combined with uniform design were used to optimized the extraction process of protein from Momordica charantia residue by salt dissolving method,and the inhibitory activity in vitroon K562 cell proliferation of crude extract was determined by MTT assay.The test results showed that the optimal conditions were as followed:salt concentration of 0.25 mol/L,time 3.59 h,pH=9,ratio of liquid-material 10.36 ∶ 1 (mL/g) and the temperature of 25 ℃,extracting twice.Under this condition,the actual extraction rate was 9.28％.In the range of experimental concentration,the crude protein from Momordica charantia residue had a significant inhibitory effect on proliferation of K562 cells in vitro,and showed dose-effect relationship.