莲壳膳食纤维微波—双酶协同提取工艺优化

摘要

以莲子壳为原料,采用微波-双酶协同提取莲壳膳食纤维,在单因素试验结果的基础上,进行正交试验,研究莲子壳筛目数、料液比、木瓜蛋白酶和糖化酶用量及提取时间对莲壳膳食纤维提取工艺的影响.结果表明:其最佳提取工艺条件为莲壳筛目数60目,液料比20∶1 (mL/g),木瓜蛋白酶加入量3％,微波协同木瓜蛋白酶酶解提取时间为6×20 s(提取6次,每次20 s,问隔2 min),糖化酶用量4％,微波协同糖化酶提取时间8×20 s(提取8次,每次20 s,间隔2 min),该条件下莲壳膳食纤维提取率为72.36％.与双酶提取法相比,微波-双酶协同提取法可缩短70％的时间,提取率提高82.87％.%To investigate the effect of microwave-two enzymes synergistic extraction methods on dietary fiber yields in lotus shell,the sieving mesh number of the lotus seed shell,ratio of solid-liquid,papain and glucoamylase dosages and extraction time were set out to conduct the orthogonal tests based on the results of single factor experiments.The optimal extraction conditions were as followed:sieving mesh of 60,ratio of liquid-solid 20 ∶ 1,papain dosage 3％,papain extr action time of 6× 20 s (extracting 6 times,each time for 20 s with intervals of 2 min),dosage of glucoamylase 4％,and glucoamylase extraction time 8 × 20s (extracting 8 times,each time for 20 s with intervals of 2 min).Under the optimal conditions,the extraction rate of dietary fiber in lotus shell was 72.36％.Compared with enzyme extraction,microwave-two enzymes synergistic extraction can save 70％ time,and greatly improve the extraction rate over 82.87％ than two enzyme method.