葛根复合固体饮料保护化学性肝损伤的研究

摘要

以葛根、山楂、酸刺、黄秋葵、干姜、玉米肽和结晶果糖为原料制备的固体饮料为受试物,研究其对Wistar大鼠CCl4所致化学性肝损伤的辅助保护作用.将Wistar大鼠随机分为阴性对照组、模型对照组和3个剂量的试验组,分别灌胃蒸馏水或1.08,2.17,4.33 g/kg bw的受试物.连续30 d后,给除阴性对照组外大鼠灌胃CCl4,成功建立化学性肝损伤模型,并测定丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)值,取肝脏称重并作病理切片,计算肝脏系数及观察肝脏的病理改变情况.相对于模型对照组大鼠,3个试验组大鼠在灌胃CCl1后,其ALT、AST水平及肝脏系数明显较低,肝组织病理改变也呈不同程度的减轻,其中以高剂量试验组的效果最为显著.因此,该受试物具有良好的辅助保护化学性肝损伤的功能.%With puerarin solid beverage made of pueraria,hawthorn,acid gill,okra,Rhizoma zingiberis,corn peptide and crystalline fructose as the test substance,to study its protection function on chemical hepatic injury by CCl4 in the Wistar mice.Wistar mice were randomly allocated to negative control group,model control group and three experiment groups,which were treated with intubation of distilled water or 1.08,2.17 or 4.33 g/kg test substance per day for 30 days.Groups which except negative control group were treated with gastric intubation of CCl4,and the chemical hepatic injury model was gained.The level of serum alanine aminotransferase (ALT),aspartate aminotransferase (AST),triglyceride (TG) were detected,and then take the liver weight and pathological section for the liver coefficients and observing the pathological changes of liver.Compared with model control group,ALT,AST and liver coefficients were decreased significantly in experiment groups after gastric intubation of CCl4,also with the mitigatory pathologic changes.And the highest dose experiment group (experiment Ⅲ) gained the most significant effects.Thus,the test substance could protect liver against chemical hepatic injury.