鸡枞菌不同溶剂提取物成分分析及抗氧化作用研究

摘要

This paper studied composition and antioxidant activity of different solvent extracts from Termitomyces albuminosus.The total flavonoids,total phenols,ergosterol and adenosine of eight kinds of different polar solvents extracts were determined by spectrophotometer and liquid chromatography.In addition,measured the antioxidant activity by scavenging DPPH ·,ABTS+ ·,hydroxyl radical,total antioxidant and total reduction ability.Extracts in distilled water had the highest rate of the yield;contained the highest content of total phenolics and adenosine in different sovlents;the highest content of ergosterol was in methanol extract.Distilled water and methanol had better scavenging free radicals in different polar solvents.The higher solvent polarity solvent was,the stronger antioxidant activity it had.The total antioxidant capacity was positively correlated with the content of the total phenolics.It has contains a variety of bioactive components,and has good anti oxidation effect in Termitomyces albuminosus.%利用分光光度法和液相色谱法测定鸡枞菌8种不同极性溶剂提取物中总黄酮、总酚、麦角甾醇及腺苷含量;通过清除DPPH·、ABTS+·、羟自由基以及总抗氧化和总还原能力来比较不同极性溶剂提取物的抗氧化活性.结果表明:不同极性溶剂提取物得率最高的为蒸馏水;蒸馏水提取物中还含有最高的总酚和腺苷;正乙烷提取物中总黄酮含量最高;甲醇提取物中麦角甾醇含量最高.蒸馏水和甲醇提取物具有更好的清除自由基效果;溶剂极性越大,抗氧化活性越强;综合抗氧化能力与总酚含量呈正相关.鸡枞菌中含有多种生物活性成分,具有很好的抗氧化作用.