酒精高浓发酵过程中果胶酶应用的研究

摘要

The viscosity reduction of broth in ethanol high-gravity-fermentation by using pectinase. The experiment results indicated that different material-to-water ratio needed different usage of pectinase were studied, The viscosity of the broth could be reduced more than 50 % when the material-to-water ratio , usage of pectinase, and reaction time were 1: 2.5, 8 u/g and 1h at 45℃ .The additional amount of the α-amylase had no influence on the viscosity of the broth.Pectinase should be added before liquefaction.%对利用果胶酶降低甘薯原料酒精高浓发酵时发酵醪的粘度进行了研究。实验结果表明：不同料水比，降低醪液粘度所需果胶酶的用量不同。料水比为1：2.5时，果胶酶(用量为8u/g(原料))在45℃下酶解料液1 h，液化后醪液的粘度可以降低55.26％；α-淀粉酶的用量对液化后醪液粘度基本无影响。添加果胶酶降解果胶应在液化之前进行。