以阿魏酸乙酯作为底物，确定了阿魏酸酯酶的酶解工艺条件：酶解时间100min；酶液与底物的体积比为0．75；提取液pH值6．2。针对阿魏酸酯酶酶学性质的研究表明：最适温度60℃，30～35℃范围内热稳定性较强。保温60min后其活力可保持在80％以上；最适反应pH值5．0，在pH值5．0—5．6范围内对阿魏酸酯酶的破坏力相对较小，保温60min其活力仍可保持在80％以上；Cu^2＋和Zn^2＋对阿魏酸酯酶有强烈的抑制作用，而ED—TA则对其有明显的促进作用。对5种小麦及其麦芽的阿魏酸酯酶酶活力进行测定：麦芽%Taking ethylferulate as substrate, the optimal enzymatic hydrolysis conditions of feruloyl esterase were confirmed as hydrolysis time 100min, the volume ratio of enzymes and substrate 0.75 mL/mL, the pH value of extract 6.2. The study on the characteristic of feruloyl esterase exhibited that the optimal temperature was 60℃ , and it had a better thermal stability between 30 -35℃, remaining over 80% of enzyme activity after insulation; the optimal pH value was 5.0, and the destruction was relatively weaker, remaining over 80% of enzyme activity after insulation; The activity was inhibited by Cu2＋ , Zn2＋ , and strongly activated by EDTA. The activity of feruloyl esterase was determined for five kinds of wheats and malts, and it was found that all of the activity of the enzyme was raised after germination in which the increase of Yan 2415, Lu Mai 21 and Zheng Mai 004 was maximum.
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