Study on Enzymatic Properties of Pectinase

摘要

In this paper, the nature of pectinase activity by an ultraviolet spectrophotometer was explored. The optimum enzyme reaction conditions of pectinase were obtained by evaluating the pectinase activity as follows: pH 9.0 and temperature at 70℃. To develop a one-step process for enzymatic desizing and scouring, the effect of amylase 2000L, cellulase, pulpzyme HC or lipase on activity of alkaline pectinase was respectively studied under optimum pectinase reaction conditions, and the effect of their mixture on pectinase activity was also studied. The results show that the pectinase retained more than 85% of its activity mixed with other enzymes. In addition, under the optimum conditions pectinase activity in different buffers was investigated to provide a theoretical support for one-bath desizing and scouring with mixed enzymes.