化学发光酶免疫法检测玉米中玉米赤霉烯酮

摘要

A sensitive indirect competitive chemiluminescence enzyme immunoassay （CLEIA） for zearalenone in corn has been developed. The optimized CLEIA gave a limit detection of 0. 09 ng/mL and a detection range of 0. 104- 1.69 ng/mL, with an IC50 of 0. 438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA. It has been validated on cereals samples in terms of precision （ intra- and interassay coefficient variations of less than 9. 1 % and 14. 7 % , respectively） and accuracy （mean recovery 79.6 % -97.6 % ）. These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples.%应用抗玉米赤霉烯酮单克隆抗体，以辣根过氧化物酶催化鲁米诺一过氧化氢化学发光体系，建立了一种简便、快速、高特异性的间接竞争化学发光酶免疫法用于检测玉米中的玉米赤霉烯酮。所建立的方法分析灵敏度为0．09ng／mL，批内变异系数小于9．1％，批问变异系数小于14．7％，样品的加标回收率为79．6％～97．6％，所建立的标准曲线相关系数为0．9884，检测线性范围为0．104—1．69ng／mL，检测时间为60min。