黏度法测定γ-聚谷氨酸含量

摘要

Abstract The viscosity of γ-polyglutamic acid (γ-PGA) solution at different concentration was determined by ubbelohde viscometer.The intrinsic viscosity [η]of γ-PGA was 7.830 L/g calculated from Huggins & Kraemer equation.Meanwhile, the correlation of relative viscosity ( ηr), specific viscosity ( ηsp ) and the γ-PGA concentration in aqueous solution was deduced.It was expected that the γ-PGA concentration could be facilely and precisely attained by the viscosity-assaying method.%采用乌氏黏度计测定了不同浓度下γ-聚谷氨酸稀溶液的黏度,由哈金斯(Huggins)方程和克拉默(Kraemer)方程结合外推法求得室温下γ-聚谷氨酸在中性水溶液中的特性黏度[η]为7.830 L/g,并推导出由溶液的相对黏度η,与增比黏度ηsp计算γ-聚谷氨酸溶液浓度的公式.依据溶液黏度与浓度的关系,可以简便、快速、准确地估算出γ-聚谷氨酸的浓度.