采用分离纯化的枯草芽孢杆菌进行产中性蛋白酶的研究,结果表明：适宜的碳源和氮源分别为玉米粉和豆粕粉;磷酸盐能提高酶产量;未添加Na2HPO4和KH2PO4的情况下,Ca2＋和Mg2＋对产酶都有抑制作用;Na2HPO4和KH2PO4存在时,1 mmol/L的Ca2＋和Mg2＋能使酶产量分别提高12%和8%,随着离子浓度的增加,产酶受到抑制;采用在11 h、18 h和24 h分别补加10 g/L玉米粉和7.5 g/L豆粕粉的补加策略,产量提高了96%,生产强度提高了112%。%Corn flour is chosen as the best carbon source and the best nitrogen source is soybean meal,using Bacillus subtilis as the neutral protease producer.Phosphate increases the protease production.When Na_2HPO_4 and KH_2PO_4 were not added in the medium,Ca~2＋ and Mg~2＋ at the given concentration cause inhibition on protease production.Ca~2＋ and Mg~2＋ at 1 mmol/L result in 12% and 8% increase in the enzyme production,respectively,with the addition of Na_2HPO_4 and KH_2PO_4 in the medium;however,higher concentration of Ca~2＋ and Mg~2＋ have an inhibitory effect on the enzyme production.Feeding of corn flour（10g/L） and soybean meal（7.5g/L） at 11,18,24 h results in 96% increase in protease yield and 112% increase in protease productivity when compared to the values obtained for batch culture.
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