claR基因的扩增对棒状链霉菌棒酸合成的影响

摘要

Clavulanic acid positive regulation gene claR of Streptomyces clavuligerus was cloned.claR expression plasmid pSET152-claR was constructed and transformed into S.clavuligerus by conjugation.The attP site in pSET152-claR could inserted into attB site of S.clavuligerus.Thus an extra copy of claR was inserted into the chromosome of S.clavuligerus.The obtained mutant S.clavuligerus： claR was cultured in the shaken flask,and the production of clavulanic acid was analyzed by HPLC,the yield of clavulanic acid was 1.86 times compared with S.clavuligerus.%从棒状链霉菌中克隆对克拉维酸具有正调控作用的基因claR。构建了claR的重组质粒pSET152-claR,通过接合转移将重组质粒pSET152-claR转入了野生型S.clavuligerus中,通过pSET152-claR中的attP位点整个质粒插入到S.clavuligerus基因组中的attB位点,实现了S.clavuligerus基因组DNA中增加一个拷贝claR基因的目的,所得突变株S.clavuligerus∷claR通过发酵培养,HPLC检测克拉维酸产量为原产量的1.86倍。