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蒸汽爆破辅助提取高温豆粕中的蛋白质

     

摘要

研究了蒸汽爆破对高温豆粕中蛋白质溶解性的影响和后续蛋白质提取工艺.结果表明:当蒸汽压力较低时,高温豆粕的氮溶解指数随着蒸汽压力的升高而提高,但当超过2.1 MPa时,压力对爆破处理的效果的影响不明显;豆粕的粒度对蒸汽爆破效果有一定的影响,当豆粕颗粒的平均小于100目时效果不佳,在20~80目时效果较好.当高温豆粕颗粒大小为20 ~ 80目,爆破条件为1.8 MPa,180 s时,蒸汽爆破处理使高温豆粕的氮溶解指数提高了1.1倍.对经蒸汽爆破处理的高温豆粕中蛋白质的提取条件研究表明,pH值和温度对蛋白质的提取率有较大影响.在优化条件下,经蒸汽爆破处理的高温粕的蛋白质提取率达到76.04%.%The effect of steam flash-explosion (SFE) on solubility and extraction of protein from thermal processed soybean meal was studied. Results obtained from the particle size and steam pressure experiments showed that when the steam pressure was low, the nitrogen solubility index ( NSI) of the soybean meal was increased with increasing steam pressure; when the particle size of soybean meal was more than 100-mesh screen, the SFE had little effect on NSI and the optimum particle size for soybean meal was between 20-mesh screen and 80-mesh screen. When steam pressure was 1. 8 MPa,180 s and the particle size of soybean meal was between 20-mesh screen and 80-mesh screen, the NSI of SFE-treated soybean meal was significantly improved by 1. 1 times than untreated soybean meal. It was found that the protein extraction was significantly influenced by pH and temperature. Under optimal conditions the maximum protein extraction yield from SFE-treated soybean meal was 76.04% .

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