马齿苋全草油的亚临界流体萃取、脂肪酸分析及抗氧化活性

摘要

以马齿苋全草油提取率为考察指标,通过单因素实验和正交实验确定亚临界异丁烷萃取技术提取马齿苋全草油的最佳提取工艺;采用气相色谱-质谱联用仪(GC-MS)分析马齿苋全草油的脂肪酸组成,同时通过羟基自由基(·OH)、DPPH·自由基和总抗氧化能力3个体外抗氧化模型评价马齿苋全草油抗氧化活性.结果表明:亚临界异丁烷萃取技术的最优工艺条件为,萃取压力0.8 MPa,萃取温度40℃,萃取时间150 min,提取率达2.91％;GC-MS分析结果表明,马齿苋全草油主要含有33.19％亚油酸(ω-6多不饱和脂肪酸)和31.04％亚麻酸(ω-3多不饱和脂肪酸);并且马齿苋全草油具有明显的总抗氧化活性并且对DPPH·和·OH具有一定的清除能力.%The technology of subcritical fluid extraction of purslane oil was optimized through orthogonal experiment based on single-factor experiment, and extraction rate was the index.The fatty acid composition of the oil was analyzed by gaschromatography-mass spectrometer(GC-MS), and in vitro antioxidant capacity of the oil was evaluated using hydroxyl radicals (· OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH ·) radicals and total antioxidant activity.The results showed that the optimum conditions of subcritical extraction were as following: extracting pressure 0.8MPa, extracting temperature 40 ℃, extracting time 150min.Under these conditions, the extraction rate of purslane oil was 2.91％.Purslane oil mainly contained 33.19％ linoleic acid(ω-6 polyunsaturated fatty acids)and 31.04％ linolenic acid(ω-3 polyunsaturated fatty acids).Results indicated that purslane oil had remarkable antioxidant activity and radical scavenging efficacy against DPPH · and hydroxyl free radical.