以水胺硫磷的特征部分为基础,设计合成半抗原O-甲基-O-2-水杨酸异丙酯硫代磷酰-6-氨基己酸(H-ICP),并通过活泼酯法将其与载体蛋白BSA、OVA分别偶联制备了免疫抗原H-ICP-BSA和包被抗原H-ICP-OVA,再经过动物免疫获得多克隆抗体.采用间接竞争ELISA法建立标准曲线,测得其IC50为5.3 ng/mL,定量检测线性为1.77～10.06 ng/mL,与其他结构类似物无交叉反应.研究所获得的抗水胺硫磷多克隆抗体,可实现果蔬中水胺硫磷农药残留快速检测.%Preparation and identification of polyclonal antibody against Isocarbophos (ICP) was studied.The hapten H-ICP was designed and synthesized on characteristics of ICP pesticide,and artificial antigens H-ICP-BSA (immmue antigen) and H-ICP-OVA (coating antigen) were prepared by coupling with the carrier proteins using active ester method.The polyclonal antibody (PAb) against ICP pesticide was produced by animal immunity.Indirect ELISA experiments showed that IC50 was 5.3 ng/mL,linearity range was from 1.77 to 10.06 ng/mL.There is no cross reaction against other structure analogous.The cloning of PAb against ICP was successfully obtained which could be used in pesticide residue detection in fruits and vegetables.
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