鸡软骨中硫酸软骨素的分离纯化

摘要

以鸡软骨为原料,对酶法提取硫酸软骨素进行纯化工艺的研究,采用阴离子交换树脂和凝胶层析法对提取的硫酸软骨素粗品进行纯化.结果表明:D218型阴离子交换树脂纯化硫酸软骨素最佳工艺条件为,吸附液用量与树脂体积比为1∶1;吸附流速为1.0mL/min;吸附时间为60 min;洗脱剂NaCl溶液的浓度为3.0mol/L;洗脱剂用量与树脂体积比为5∶1,最佳解析时间为75 min,硫酸软骨素纯度为72.03％.通过葡聚糖凝胶G-75纯化后得到的硫酸软骨素的纯度为99.30％,较第一次纯化提高了27.27％.通过高效液相色谱法对纯化后的物质进行检测,进一步证明从鸡软骨中提取的物质为CS.%The anion exchange resin and gel chromatography method was used to purification chondroitin sulfate.The anion exchange resin D218 was selected to purify chondroitin sulfate by the way of static adsorption and analysis experiments.The best purification conditions of chondroitin sulfate was determined through dynamic adsorption and analysis was as follows:The rate of adsorption amount of liquid and resin volume was 1 ∶ 1,the adsorption velocity was 1.0 mL/min,the adsorption time was 60 min,the eluant of NaCl solution was 3.0 mol/L,the amount of eluant and resin volume ratio of 5 ∶ 1,the analysis time was 75 min,the purity of chondroitin sulfate was 72.03 ％.SephadexG75 was selected to purify chondroitin sulfate again,,the purity of chondroitin sulfate was 99.30％ under this condition,compared with the first purification improved 27.27％.The result showed that the substance was CS that was extracted from chicken cartilage through the method of HPLC.