L-serine is a metabolite in the central metabolic pathway and involves the intracellular essential C-1 unit cycle.Its partial branch pathways for degradation cannot be deleted directly,which greatly hampers strain improvement for L-serine accumulation by traditional methods.Current method for L-serine production mainly depends upon precursor conversion by C-1 compound utilizing microorganisms.With rapid development of modern biotechnology,researches refocus on the previous attempts to produce L-serine from raw sugar materials and improve its productivity and yield by metabolic engineering.The review will elaborate the recent advancement on microbial production of L-serine and discuss the problems about genetically engineering of the central metabolic pathway of serine biosynthesis.%L-丝氨酸作为胞内中心代谢产物,参与了胞内重要的C-1活性单元循环,其降解代谢的部分分支途径不能被直接敲除,与其他氨基酸相比,微生物法制备难度较大.以往采用传统的育种方法均难以获得高产L-丝氨酸的菌株,目前的制备方式以微生物前体转化为主.近几年,随着现代生物技术的快速发展,胞内代谢通量的实时分析和遗传改造技术不断增强,人们开始重新关注从糖质原料合成L-丝氨酸的育种研究,并应用多种代谢工程策略改造菌株.该文从L-丝氨酸作为中心代谢产物的特征、微生物前体转化的制备技术以及从糖质原料合成丝氨酸的代谢工程改造3个方面进行阐述,分析微生物法制备L-丝氨酸的相关进展以及存在问题.
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