水不溶性条斑紫菜多糖酸水解的研究

摘要

小分子多糖水解产物能够克服大分子多糖溶解性差、生物利用率低的不足.文中对水不溶性条斑紫菜多糖的盐酸水解进行研究.结果表明,盐酸水解多糖的最适条件为,酸浓度0.050 mol/L,温度80℃,时间2.0h.水解产物中还原糖占总糖的0.24％,低聚糖产物可能为二糖或三糖.产物的红外光谱分析表明粉状样品是含硫酸基的β-糖苷键连接的吡喃型低聚糖,其具有糖环内醚结构.产物的紫外光谱分析表明粉状样品含有蛋白质,进一步分析表明样品含有蛋白质6.88％、总糖88.92％.%Small molecular weight hydrolysates from polysaccharide hydrolysis are better than big molecular weight polysaccharides on the solubility and bioavailability.In this work,the hydrolysis of water-insoluble polysaccharides from Porphyra yezoensis was investigated using hydrochloric acid method.The optimal conditions for hydrolyzing polysaccharides were determined as:acid concentration 0.050 mol/L,80 ℃ reacted for 2.0 hours.In the hydrolysates,the ratio of reducing sugar to total sugar was 0.24％,and the oligosaccharide product could be disaccharide or trisaccharide.The infrared spectrum of powder hydrolysates showed they were pyranoses with sulphuric groups,β-glucosidic bonds and cyclic ether structure.The ultraviolet spectrum of powder hydrolysates showed they contained proteins,and further analysis demonstrated the powder hydrolysates contained 6.88％ protein,and 88.92％ total sugar.