温度和盐度对华贵栉孔扇贝幼贝淀粉酶和 Na + /K +-ATP 酶活性的联合效应

摘要

The synergistic effects of temperature (19—31 ℃) and salinity (22—38) on activities of amylase and Na+ /K+-ATPase were studied in juvenile scallop Chlamys nobilis with shell length of (7 .52 ± 0 .12) mm by two-factor central composite design (CCD) and response surface methodology (RSM ) .There were significant linear and quadratic effects of temperature on the activities of amylase and Na + /K+-A T Pase ( P<0 .05) .The significant linear effect of salinity on the activity of Na + /K+-ATPase was found (P<0 .05) ,without significant linear effect of salinity on the activity of amylase (P>0 .05) ,and the significant quadratic effects of salinity on the activities of amylase and Na + /K+-ATPase were observed (P<0 .05) . There were significant synergistic effects of both temperature and salinity on the activity of amylase (P<0 .05) ,and no significant synergistic effects of both temperature and salinity on the activity of Na + /K+-ATPase were observed . The activities of amylase and Na+ /K+-ATPase were inhibited under high temperature and salinity ,with gradual decline in activities of amylase and Na + /K+-ATPase .A model equation about the relationship between the activities of amylase and Na + /K+-ATPase and the two factors was established by response surface methodology ,with the r2 as high as 0 .9815 in temperature and 0 .9582 in salinity , indicating that the satisfactory fitting capability of the model was practicably applied for prediction .The optimization of the reliable model revealed that the maximum activities of amylase (0 .4809 U/mg) and Na+ /K+-ATPase (0 .0382 U/mg) ,with the desirability value of 0 .910 ,were observed at the 2-factor combination of water temperature of 25 .60 ℃ and a salinity of 30 .67 .%采用中心复合设计和响应曲面法 ,研究了温度(19~31 ℃)和盐度(22~38 )对华贵栉孔扇贝幼贝淀粉酶和Na+ /K+-ATP酶活力的联合效应.试验结果表明 ,温度对淀粉酶和Na+ /K+-ATP酶活力的一次效应和二次效应影响显著(P<0 .05);盐度对Na+ /K+-ATP酶活力的一次效应影响显著(P<0 .05) ,但对淀粉酶活力影响不显著(P>0 .05);盐度对淀粉酶和Na+ /K+-ATP酶活力的二次效应影响显著(P<0 .05).温度和盐度对淀粉酶活力影响存在互作效应 ,且影响显著(P<0 .05) ,但温度和盐度对Na+ /K+-ATP酶活力影响不存在互作效应.温度和盐度过高时 ,抑制Na+ /K+-ATP酶和淀粉酶的活力.采用响应曲面法建立了温度和盐度对淀粉酶和Na+ /K+-ATP酶活力影响的模型方程 ,决定系数分别为0 .9815和0 .9582 ,表明模型的拟合度极高 ,可用于预测华贵栉孔扇贝幼贝中淀粉酶和Na+ /K+-A T P酶活力的变化.通过模型优化和验证试验表明 25 .60 ℃和盐度30 .67时 ,淀粉酶和Na+ /K+-ATP酶活力达最大值 ,分别为0 .4809 U/mg和0 .0382 U/mg ,满意度为0 .910.