HCCR mRNA在原发性肝细胞癌患者中的表达及临床意义

摘要

Objective To detect the expression level of HCCR mRNA in primary hepatocellular carcinoma(PHC) and explore its clinical significance. Methods Reverse transcription polymerase chain reaction was used to detect the expression of HCCR mRNA in PHC tissues, surrounding non-tumor tissues, hepatocirrhosis tissues and peripheral blood mononuclear cells. Results The relative expression level of HCCR mRNA in PHC tissues was 0.83±0.10, and which in surrounding non-tumor tissues and the hepatocirrhosis tissues were 0.12±0.07 and 0.57±0.12, respectively. The differences in the three groups had statistical significance (P<0.05). The relative mRNA level of HCCR in the PBMC of PHC patients were 0.55 ±0.06 and which in the hepatocirrhosis pa-tients were 0.34±0.04, while HCCR expression in the normal ones could not detected. The differences had statistical significance (P<0.05). Conclusion The mRNA levels of HCCR in PHC tissues, hepatocirrhosis tissues and their PBMC are up-regulated sig-nificantly, which suggests that HCCR maybe involved in the occurrence and development of PHC, and maybe related to the ma-lignant change of PHC.%目的：探讨人宫颈癌基因(human cervical cancer oncogene，HCCR) mRNA在原发性肝细胞癌(primary hepatocellular carcinoma，PHC)患者中的表达及其临床意义。方法采用RT-PCR检测PHC癌组织、癌旁肝组织和肝硬化组织及各组外周血单个核细胞(peripheral blood mononuclear cell，PBMC)中的HCCR mRNA表达水平，并进行分析。结果 HCCR mRNA 在PHC组织中的相对表达强度为0.83±0.10，癌旁肝组织中的相对表达强度为0.12±0.07，肝硬化组织中的相对表达强度为0.57±0.12，三组均数间两两比较，差异均有统计学意义(P<0.05)。 PBMC中，肝癌患者HCCR mRNA的表达强度为0.55±0.06，肝硬化患者HCCR mRNA的表达强度为0.34±0.04，而正常人未检测出，差异均有统计学意义(P<0.05)。结论 PHC和肝硬化患者的组织和PBMC中HCCR表达明显增高，HCCR基因可能参与了PHC的发生与发展，其与PHC的恶变演进有一定的相关性。