基于Tenax脱附技术评价洋山港海域悬浮物中多环芳烃的生物有效性

摘要

The concentrations of polycyclic aromatic hydrocarbons (PAHs) in marine organisms were measured to calculate the biota-sediment accumulation factor (BSAF) of suspend particulates from Yangshan port.Desorption kinetics of PAHs in suspend particulates using Tenax was described and proposed as an efficient way to assess the bioavailability of PAHs.The results showed that the BSAFs of PAHs ranged from 0.02～0.21,suggesting the low ability of PAHs bioaccumulation in marine organisms.Desorption kinetics experiments using Tenax indicated that the Frap was from 20％～40％ and the order of the rate constants for the three desorption compartments (fast,slow and very slow) were 10-1,10-2 and 10-4,respectively.Furthermore,the correlations between the concentrations of PAHs in Tenax 6 h and those of the marine organisms were significant (0.64＜r2＜0.80,P＜0.01),which indicated that Tenax 6 h might be a feasible way to predict body residual of PAHs in marine organisms and represent a more efficient and rapid way to measure the bioavailable fraction of PAHs compared with the traditional method.Moreover,it can also be used to evaluate the pollution of PAHs in the port area and to provide basis for the assessment of the quality of seafood and relevant health risk via consuming seafood.%在测定海洋生物对洋山港海域悬浮物中多环芳烃(Polycyclic Aromatic Hydrocarbons,PAHs)富集系数的基础上,结合Tenax连续萃取法研究悬浮物中PAHs的脱附动力学,为评价洋山港悬浮物中PAHs的生物有效性提供简单、快速的评价方法.结果表明,洋山港海域生物对悬浮物中PAHs的生物-底泥富集因子(BSAF)处于0.02～0.21之间,说明该海域生物体对悬浮物中PAHs的富集程度较低;悬浮物中PAHs的快速脱附比例(Frap)处在20～40％之间,且快速、慢速和极慢速脱附速率常数数量级分别为10-1、10-2和104;此外Tenax6 h单点萃取的PAHs浓度与大多数海洋生物体内的累积浓度相关性良好(0.64＜r2＜0.80,P＜0.01),表明Tenax 6 h单点萃取比例可以简便、快速地预测悬浮物中PAHs在海洋生物体内的累积量.该方法可用于评价海域悬浮物PAHs的污染并为相关海产品的质量安全和食用风险提供生物有效性评价依据.