2007-2011年北京市通州区分离单增李斯特菌的生物学及分子流行病学特征

摘要

Objective To understand the biological and molecular epidemiological characteristics of Listeria monocytogenes strains isolated in Tongzhou from 2007 to 2010, and provide evidence for the prevention and control of listeriosis. Methods Detection of 6 virulence factors (prfA, actA, iap, hly, plcB, inlA) of L. monocytogenes were conducted using PCR and the strains were characterized by serotyping and PFGE typing. Results The 6 virulence factors were detected in 57 L monocytogenes strains. The strains belonged to 5 serotypes and 19 PFGE patterns. The predominant serotypes were l/2a and l/2c and the major PFGE pattern was GX6A16. CN0004. Conclusion All the L. monocytogenes strains isolated in Tongzhou carried 6 virulence factors and showed polymorphism in molecular type. According to the study result, it is necessary to strengthen the surveillance of L monocytogenes.%目的 了解2007 -2011年北京市通州地区食品及患者分离的单增李斯特菌的生物学和流行病学特征,为李斯特菌病的预防控制提供参考依据.方法 对分离的单增李斯特菌进行6个毒力因子(prfA、actA、iap、hly、plcB、inlA)的检测、血清分型和脉冲场凝胶电泳分型.结果 57株单增李斯特菌的6个毒力因子均为阳性,分为5种血清型,主要血清型为1/2a和1/2c型,PFGE分型分为19种带型,主要带型为GX6A16.CN0004.结论 通州地区分离的单增李斯特菌全部携带6个主要毒力因子,分子型别呈现多态性.