抗根肿病大白菜的花药培养

摘要

Chinese cabbage is one of the important vegetables in northern China. Clubroot disease seriously influenced the production of Chinese cabbage in the world. The use of anther culture can greatly accelerate breeding process of Chinese cabbage resistant to c%大白菜是中国北方的重要蔬菜之一，根肿病是危害大白菜生产世界性病害，利用花药培养可以大大加速抗根肿病大白菜杂交育种工作的进程。对33份抗根肿病大白菜品种（品系）进行花药培养，有24个品种诱导出胚，品种诱导率为72．7％，以东方皇冠×C11的诱导率最高，为1．86胚／蕾。对大部分基因型来说，在培养基中添加0．4g／L谷氨酰胺的诱导效果最好。研究还发现生长在温室中的供体植株更容易诱导出胚状体。变绿的子叶型胚转到B5＋0．2mg／LBA＋0．1mg／LNAA＋0．1％活性炭的胚分化培养基上可诱导生芽。