腹泻型肠易激综合征患者粪便双歧杆菌/肠杆菌值与血清5-羟色胺、直肠最大耐受阈值相关性研究

摘要

Objective To explore the variety of the Biifdobacterium and E.coli value and the 5-hydroxytryptamine (5-HT) and rectal maximum tolerable threshold in the Diarrhea-predominant irritable bowel syndrome patients and health man ,and the association of the Biifdobacterium and E.coli value and the 5-HT and MTV. Methods 31 cases of Diarrhea-predominant irritable bowel syndrome patients subjects were experimental group. 30 normal health people were as control group. To exmine the 5-hydroxytryptamine (5-HT) by using the lfuorescence spectrophotometer, to examine the Biifdobacterium and E.coli value by using real time lfuorescence quantitative PCR-DNA/RNA detection, to examine MTV by using gastrointestinal function monitor. Results There were signiifcantly differences in Biifdobacterium and E.coli value and the 5-H and MTV between in 2 groups. Biifdobacterium and E.coli value has correlated negatively with the 5-HT, positively with MTV. Conclusion For Diarrhea-predominant irritable bowel syndrome patients the Biifdobacterium and E.coli value and the 5-HT and MTV are important roles in the develepment of Diarrhea-predominant irritable bowel syndrome. If lift the level of Biifdobacterium and E.coli value, to improve lfora imbalance, the 5-HT maybe reduced, MTV maybe improved .%目的：探讨腹泻型肠易激综合征(IBS-D)患者粪便双歧杆菌/肠杆菌（B/E）值与5-羟色胺(5-HT)、直肠最大耐受阈值（MTV）的相关性。方法检测试验组腹泻型肠易激综合征患者（n=31）与正常对照组健康志愿者（n=30）粪便中B/E值，以及血清中5-HT的含量，直肠MTV。测定血清5-HT用荧光分光光度计法，粪便B/E值用PCR-DNA/RNA实时荧光定量检测测定，胃肠功能监测仪测定直肠MTV。结果2组粪便中B/E值及血清中5-HT的含量、直肠MTV比较差异均有统计学意义（P<0.05）。粪便B/E值与血清5-HT水平呈负相关，与直肠MTV成正相关。结论腹泻型肠易激综合征患者直肠MTV、血清中5-HT水平升高与肠道菌群失调严重程度有关。改善菌群失调可能降低5-HT水平，升高直肠MTV。