气相色谱-质谱法测定化妆品中维生素E

摘要

建立了气相色谱-质谱联用法(GC-MS)同时检测化妆品中α-生育酚和α-生育酚醋酸酯的分析方法.样品采用乙腈水溶液-正己烷分散提取,以HP-5MS色谱柱分离,GC-MS选择离子监测模式测定.实验表明,α-生育酚和α-生育酚醋酸酯在0.05 ～ 10 mg/L范围内呈良好的线性关系,相关系数均大于0.999;此方法检出限(S/N=3)分别为0.6和0.5 mg/kg,定量限(S/N=10)分别为1.9和1.8 mg/kg;阴性样品的2个添加水平的平均回收率在88.7％～ 106.3％之间,相对标准偏差(RSD,n=6)小于8.2％.%A sensitive method was established for the determination of alpha-tocopherol and alpha-tocopheryl acetate in cosmetics using gas chromatography-mass spectrometry (GC-MS).Sample were dispersed and extracted with acetonitrile water solution-n-hexane.Then the n-hexane layer was separated by HP-5MS capillary chromatographic column and detected by selected ion monitoring (SIM) mode of GC-MS under the optimized condition.The results showed that the calibration curves of target analytes were linear in the range of 0.05～10 mg/L with correlation coefficients higher than 0.999.Detection limits (LODs,S/N =3) of alpha-tocopherol and alpha-tocopheryl acetate were 0.6 mg/kg and 0.5 mg/kg,respectively,and quantification limits (LOQs,S/N=10) were 1.9 mg/kg and 1.8 mg/kg,respectively.Mean recoveries of 76.3％～99.7％ for two concentrations spiked were achieved,the relative standard deviations (RSD,n=6) were less than 8.2％.