UHRF1蛋白在胃癌组织中表达及其与胃癌患者临床病理特征关系

摘要

目的 探讨UHRF1蛋白在胃癌组织中的表达及其与胃癌患者临床病理特征的关系.方法 采用反转录-聚合酶链反应(RT-PCR)及免疫组织化学方法分别检测胃癌102例胃癌组织和癌旁组织中UHRF1的mRNA及蛋白表达水平,并分析UHRF1蛋白表达与胃癌患者临床病理特征关系.结果 RT-PCR及免疫组织化学检查结果显示胃癌患者胃癌组织中UHRF1 mRNA及蛋白表达水平均显著高于其癌旁组织,差异均具有统计学意义(P<0.05).UHRF1蛋白表达与胃癌患者的某些病理特征显著相关,在UHRF1蛋白表达量较高的胃癌患者中,其肿瘤直径较大、TNM临床分期较晚、浸润程度较深,且出现淋巴结转移概率较大、组织分化程度也较高.结论 UHRF1在胃癌组织中呈高表达,并与肿瘤恶性表型相关,可作为判断胃癌发生和发展的重要辅助指标.%Objective To investigate UHRF1 protein expression in gastric cancer tissues and its relationship with clinicopathological characteristics of gastric cancer.Methods The mRNA and protein expressions of UHRF1 in cancer and ambient tissues were detected in 102 patients with gastric cancer by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry methods respectively, and the relationship between UHRF1 protein expression and clinicopathological characteristics was analyzed.Results RT-PCR and immunohistochemistry results showed that mRNA and protein expressions of UHRF1 in gastric cancer tissues were significantly higher than those in ambient tissues (P<0.05).UHRF1 protein expression had significant relationship with some clinicopathological characteristics.Gastric cancer tissues of higher UHRF1 protein expression had larger tumor diameter, later TNM staging, deeper infiltration depth, higher incidence rate of lymph node metastasis and histological differentiation degree (P<0.05).Conclusion UHRF1 is highly expressed in patients with gastric cancer, and it is closely correlated with malignant phenotype of gastric cancer, and therefore it can be used as an important assistance index for judging occurrence and development of gastric cancer.